Applications
Analysis of Cell Ploidy Utilizing High-Content Screening Techniques
Cell populations that are actively dividing contain cells with 2X or even greater multiples of the normal diploid DNA content. Analysis of DNA content is very actively pursued in biomedical research…
Affymetrix Quantigene® ViewRNA novel mRNA quantification technology
New method to quantify and visualize individual mRNA molecules within cells All gene expression begins with transcription. As the process of transcribing messenger RNAs from the…
Analysis of Cadherin Expression in Cultured Cells and Tissue Sections (Z’ > 0.5)
Cadherins proteins are integral to the plasma membrane and function in cellular adhesion, both in tissues and in cultured cells. The cadherin superfamily of proteins contains…
Analysis of VE-cadherin Expression in Primary Human Endothelial Cells (Z’ > 0.5)
VE-cadherin (vascular endothelium cadherin, also known as Cadherin-5), is a member of cadherin superfamily, expressed by vascular endothelial cells (reviewed in 1-3). In these cells it is the…
DNA Ploidy with Tissue Sections and Cadherins in HT Imaging
There is growing demand for obtaining information from tissue slices collected during research, clinical, or pathological studies. Information on DNA content per cell (ploidy) is of particular…
Measuring Caspase 3 Activation Using CyteSeer’s Protein Expression Algorithm
Introduction: Apoptosis is a programmed series of events that leads to the removal of old, unwanted, or damaged cells from the tissues of multicellular organisms [1]. In contrast…
Monitoring Activation of Protein Kinase C-alpha via HT Imaging (Z’ > 0.5)
An essential process of drug discovery is testing candidate pharmaceuticals on cells for potential therapeutic or toxic effects. Many drug discovery companies have instituted high throughput…
Monitoring Distribution of N-Cadherin and E-Cadherin with HT Imaging
Introduction: N-cadherin and E-cadherin are widely distributed cell adhesion molecules that feature large extracellular domains which bind to each other on neighboring cells in a zipper-like…
Monitoring Expression and Distribution of B-Catenin (Z’ > 0.5)
Introduction: m-catenin, the mammalian homologue of Drosophila Armadillo is a dual purpose protein of great interest to those biomedical researchers concerned with tumor formation. In…
Perilipin and Phosphoperilipin Control of Lipolysis
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Quantification of Acetylated Histones (Acetyl-H3) (Z’ > 0.5)
Introduction: Histones are small proteins that bind to DNA and are involved in chromosome packing and the regulation of gene transcription. In the basal…
Quantify Lipid Droplets and Associated Proteins in Human Adipocytes
In 2002, Surgeon General David Satcher stated that “...obesity may soon cause as much preventable disease and death as cigarette smoking” (FDA website: http://www.fda.gov/fdac/features…
Quantify Lipid Droplets in Hepatocytes
Fatty Liver Disease Fatty liver disease (FLD), also known as non-alcoholic steatohepatitis (NASH), recently came to light as a condition which afflicts an estimated 20 to 35% of…
Vala Forums
Vala's Forums section is another great place to request and find more information about using CyteSeer and other Vala kits in the…
