RNA Spots Measurement Reference
RNA In Situ Hybridization spot measurements for use with the Affymetrix/Panomics ViewRNA Kits. This algorithm will perform well as a general FISH analysis tool.
RNA Spots
Short Name <--------------> Long Name
Cell ID <--------------> ID of each record
1 Well <--------------> Name of the well
2 RNA-1 Spot Count <--------------> Number of RNA-1 Spots
3 RNA-1 Area <--------------> RNA-1 Area
4 RNA-1 Intensity <--------------> Total Integrated Intensity of RNA-1
5 RNA-1 Nuclear Spot Count <--------------> Number of RNA-1 Spots in Nucleus
6 RNA-1 Nuclear Spot Count Density <--------------> RNA-1 Spot Density in Nucleus
7 RNA-1 Area in Nucleus <--------------> RNA-1 Area in Nucleus
8 RNA-1 Area Density in Nucleus <--------------> RNA-1 Area Density in Nucleus
9 RNA-1 Intensity in Nucleus <--------------> Total Integrated Intensity of RNA-1 in Nucleus
10 RNA-1 Intensity Fraction in Nucleus <--------------> RNA-1 Intensity in Nucleus as Ratio of Nuclear Intensity
11 RNA-2 Spot Count <--------------> Number of RNA-2 Spots
12 RNA-2 Area <--------------> RNA-2 Area
13 RNA-2 Intensity <--------------> Total Integrated Intensity of RNA-2
14 RNA-2 Nuclear Spot Count <--------------> Number of RNA-2 Spots in Nucleus
15 RNA-2 Nuclear Spot Count Density <--------------> RNA-2 Spot Density in Nucleus
16 RNA-2 Area in Nucleus <--------------> RNA-2 Area in Nucleus
17 RNA-2 Area Density in Nucleus <--------------> RNA-2 Area Density in Nucleus
18 RNA-2 Intensity in Nucleus <--------------> Total Integrated Intensity of RNA-2 in Nucleus
19 RNA-2 Intensity Fraction in Nucleus <--------------> RNA-2 Intensity in Nucleus as Ratio of Nuclear Intensity
20 Cell Area <--------------> Cell Area
21 Nucleus Area <--------------> Nucleus Area
22 Nucleus Intensity <--------------> Total Integrated Nucleus Intensity
23 X-Nucleus <--------------> X-Position of Nucleus
24 Y-Nucleus <--------------> Y-Position of Nucleus
25 Boundary Cell <--------------> Boundary Cell
