Reagents and Kits - Vala Sciences

Vala Reagents and Kits

Vala Sciences Screen-Certified (Z'> 0.5) Cell-Imaging Kits include fixative, permeabilization, and staining solutions in amounts sufficient to stain cells cultured in five 96-well dishes.

b-catenin Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$750

b-catenin, the mammalian homologue of Drosophila Armadillo, is a dual purpose protein of high interest to biomedical researchers concerned with tumor formation. In normal epithelial cells, b-catenin is found at the plasma membrane. b-catenin expression and localization can be quantified in cultured cells with Vala Sciences Inc.'s b-catenin kit.

E-Cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$700

E-cadherin, a cell-cell adhesion molecule, is frequently downregulated in a wide variety of tumors (breast, skin, prostate, gastric, and colon cancers), suggesting that it is an important tumor suppressor. E-cadherin is the major binding partner for -catenin, and E-cadherin downregulation is often accompanied by beta-catenin translocation to the nucleus, and the stimulation of cell division. E-cadherin expression and localization can be quantified in cultured cells with Vala Sciences Inc's E-cadherin kit.

Lipid Droplet Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$220

Lipid droplets are the primary fat storage organelles of fat cells (adipocytes). Lipid droplet size, and number are altered by anti-diabetic and anti-obesity drugs and these effects can be quantified utilizing Vala Science Inc's Lipid Droplet Kit. The kit can also be utilized to quantify lipid droplets in murine 3T3 L1 adipocytes.

N-Cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$435

N-cadherin, a cell-cell adhesion molecule, is upregulated in many tumor types (including breast, ovarian, prostate, and skin cancers) and may be involved in metastastic potential. N-cadherin often replaces E-cadherin during tumorigenesis, a phenomenon referred to as the "cadherin switch". N-cadherin expression and cellular localization can be quantified in cultured cells with Vala Sciences Inc's N-cadherin kit.

Pan-Cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5, Mouse mAb-488)

$380

Accurate quantitation of cadherin expression and localiztion as they are related to cell adhesion, tissue structure and mitotic signaling are valuable across a variety of biomedical contexts including cell differentiation, embryonology, neurobiology, vascular pharmacology, and oncology. Vala Sciences' Pan-cadherin assay kits, used in conjunction with Vala's CyteSeer® automated cell image analysis software, enable precise quantification of cadherin expression and localization.

Pan-Cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5, Mouse mAb-547)

$260

Pan-Cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5, Rabbit Poly-488)

$300

Pan-Cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5, Rabbit Poly-547)

$300

Perilipin Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$950

Perilipin is a protein that associates with lipid droplets in mature adipocytes and is involved in lipid droplet metabolism. The association of perilipin with lipid droplets in primary human adipocytes can be quantified through use of Vala Science Inc's Perilipin kit.

PKC-Alpha Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$340

Protein kinase Cα(PKCα), a key regulatory protein in signal transduction pathways modulating cancer and inflammation, is activated via G-protein coupled receptors, tumor promoting chemicals, and other stimuli. Activation of PKCa depends upon migration of PKCa from the cytoplasm to the cell membrane. The expression and activation of this important signal transduction molecule can be quantified in cultured cells (validated for HeLa, UMR106, or IEC18 cells), with Vala Sciences Inc's PKCa kit.

VE-cadherin Screen-Certified Cell-Imaging Kit (Z’ > 0.5)

$550

VE-cadherin is expressed by vascular endothelial cells where it regulates cell-cell adhesion, angiogenesis, and vascular permeability. VE-cadherin is upregulated in breast cancer and may participate in tumor-associated angiogenesis. VE-cadherin expression and localization can be quantified in cultured cells with Vala Sciences Inc's VE-cadherin kit.

Definition of Z’: For high throughput assay development and validation, a statistic has been defined (Z’) which provides a quantitative index of the relationship between the sample to sample variability and the range of the assay (Zhang et al., 1999). Z’ = 1-3(SDmax+SDmin)/(Xmax-Xmin) where SDmax and SDmin are the standard deviations of the maximum and minimum value samples, respectively, and Xmax and Xmin are the means of the maximum and minimum, respectively.

Z’ has a theoretic range of negative infinity to 1.0 (an ideal assay in which there is no standard deviation and finite separation between means would yield Z’ = 1.0). A positive Z’ score is much more stringent than a p < 0.05 determination from a t-test. Z’ scores > 0.5 are excellent and indicate that an there is an extremely low probability of obtaining ‘false positives’ such assays can be used, with very high confidence, to screen libraries representing hundreds of thousands of test compounds.

Zhang, J.H., Chung, T.D. & Oldenburg, K.R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 4, 67-73 (1999).

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