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    <title type="text">Vala Sciences</title>
    <subtitle type="text">Keep track of the latest news and product updates from Vala Sciences</subtitle>
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    <updated>2009-02-14T20:26:25Z</updated>
    <rights>Copyright (c) 2010, Vala Sciences</rights>
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    <entry>
      <title>Display Gated Images Live in CyteSeer</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/news/id/display-gated-images-live-in-cyteseer/" />
      
      <id>tag:valasciences.com,2010:vs/news/archives/2.199</id>
      <published>2010-07-30T17:55:42Z</published>
      <updated>2010-07-30T19:40:38Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <content type="html"><![CDATA[
                See which cells remain with new Display Gated Images panel.
        If you&#39;re a CytoShop or CyteSeer user, you&#39;ll&amp;nbsp;want to take a look at the new &quot;Display Gated Image&quot; in the data viewer under the well data tab. It&#39;s pretty amazing. Adjust a gate and see which cells remain in the field. The others are still there but are shaded. A great way to know you are making a measurement over the correct population of cells.

&amp;nbsp;
CyteSeer is available now on the download page.
      ]]></content>
    </entry>

    <entry>
      <title>New Anti&#45;phospho&#45;perilipin Mouse Monoclonal Antibody (Serine 522)</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/news/id/new-anti-phospho-perilipin-mouse-monoclonal-antibody-serine-522/" />
      
      <id>tag:valasciences.com,2010:vs/news/archives/2.195</id>
      <published>2010-07-29T23:00:58Z</published>
      <updated>2010-07-29T23:06:00Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <content type="html"><![CDATA[
                Another new antibody! 
        We&#39;ve just released another new phospho&#45;perilipin antibody. We&#39;re excited about the growing family of lipid relevant antibodies we have available. One derived from a site near S522 (Vala#4856) with another near S497 (Vala#4855). These two phospho flavors complement that of the perilipin A (Vala#4854) and all three recognize the full length Perilipin A.
Big thanks to all of our collaborators and early customers. The feedback and general excitement has been great.
      ]]></content>
    </entry>

    <entry>
      <title>Anti&#45;phosphoperilipin Mouse Monoclonal Antibody (Serine 522)</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/reagents" />
      
      <id>tag:valasciences.com,2010:vs/reagents/3.194</id>
      <published>2010-07-23T15:08:44Z</published>
      <updated>2010-07-30T17:09:45Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

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    </entry>

    <entry>
      <title>New Perilipin and Phosphoperilipin mAbs</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/news/id/new-perilipin-and-phosphoperilipin-mabs/" />
      
      <id>tag:valasciences.com,2010:vs/news/archives/2.193</id>
      <published>2010-04-22T22:04:44Z</published>
      <updated>2010-04-22T22:07:45Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <content type="html"><![CDATA[
                Vala&#39;s two new monoclonal antibodies for visualizing perilipin in immunocytofluorescence labeling applications
        &amp;nbsp;



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Obesity is an overwhelming problem in the USA and in developing countries, worldwide(1, 2). The abundance of food and lack of exercise leads to an accumulation of triglycerides within lipid droplets in fat tissue (adipocytes). In adipocytes, the lipid droplets are coated with a layer of proteins that regulate their growth and metabolism. The major lipid droplet&#45;associated protein in adipocytes is perilipin(3). Mice in which the endogenous gene for perilipin is knocked out have a lean phenotype due to reduced accumulation of fat(4, 5), suggesting that perilipin may have a &amp;ldquo;gate&#45;keeper&amp;rdquo; function under basal conditions to protect the triglycerides from metabolism by lipases. Conversely, perilipin is subject to phosphorylation by cyclic&#45;AMP&#45;dependent protein kinase, and phosphorylation of perilipin helps to recruit Hormone Sensitive Lipase (HSL) to the lipid droplets, an important step in the initiation of lipolysis (Figure 1A).



Figure 1.&amp;nbsp;A, Human adipocytes visualized for nuclei (blue), lipid droplets (green), and perilipin (red). B, Lipolysis in adipocytes is regulated by perilipin (circles), hormone sensitive lipase (HSL, triangles), and other regulatory proteins (e.g., ATGL, not shown). A well known pathway, depicted here, is activation of lipolysis by&amp;nbsp;&amp;Icirc;&amp;sup2;&#45;adrenergic stimulation. Interaction of isoproterenol (Iso) with&amp;nbsp;&amp;Icirc;&amp;sup2;&#45;adrenergic receptors (BAR) leads to increased cAMP, and activation of c&#45;AMP&#45;dependent protein kinase (PKA). PKA phosphorylates both perilipin (filled circles) and HSL and phosphoHSL (filled triangles), leading to translocation of HSL to the lipid droplets.



Given the ever increasing interest in elucidating the mechanisms that regulate lipolysis, Vala Sciences Inc is proud to introduce two new monoclonal antibodies that are excellent at visualizing perilipin in immunocytofluorescence labeling applications. The first antibody (Figure 1B) recognizes perilipin whether or not lipolytic pathways have been activated in the cell. Labeling with the antibody is exceptionally specific, with virtually no background, and the labeling pattern is very tightly associated with the outer surface of the lipid droplets, as expected for perilipin.




Figure 2. Assay of phorphorylated perilipin via immunocytofluorescence.&amp;nbsp;Human subcutaneous adipocytes labeled for nuclei (blue), lipid droplets (green), and perilipin (red), using Vala Sciences&amp;rsquo; phospho&#45;perilipin specific monoclonal antibody. A, Cells exposed to control medium. B, Cells exposed to 10 &amp;micro;M FSK + 500 &amp;micro;M IBMX for 2 minutes.



The second monoclonal antibody selectively recognizes perilipin that has been phosphorylated on serine 497, a site of PKA&#45;mediated phosphorylation (Figure 2). Thus, for the first time, a tool is available in which phosphorylated perilipin can be visualized in fixed cells via immunocytofluorescence labeling procedures and fluorescence microscopy. This antibody is expected to be of high interest to researchers investigating hormonal control of lipolysis.






&amp;nbsp;
      ]]></content>
    </entry>

    <entry>
      <title>Perilipin and Phosphoperilipin Control of Lipolysis</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/articles/applications/perilipin-and-phosphoperilipin-control-of-lipolysis" />
      
      <id>tag:valasciences.com,2010:articles/f/4.192</id>
      <published>2010-04-13T22:56:38Z</published>
      <updated>2010-04-13T23:05:40Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <category term="Applications"
        scheme="http://www.valasciences.com/site/C4/"
        label="Applications" />
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    </entry>

    <entry>
      <title>Anti&#45;phosphoperilipin Mouse Monoclonal Antibody (Serine 497)</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/reagents" />
      
      <id>tag:valasciences.com,2010:vs/reagents/3.191</id>
      <published>2010-04-13T22:33:04Z</published>
      <updated>2010-07-30T17:10:06Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

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    </entry>

    <entry>
      <title>Anti&#45;perilipin Mouse Monoclonal Antibody (Serine 497)</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/reagents" />
      
      <id>tag:valasciences.com,2010:vs/reagents/3.190</id>
      <published>2010-04-13T22:21:27Z</published>
      <updated>2010-07-30T17:10:28Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

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    </entry>

    <entry>
      <title>Manders Coeffients in CyteSeer</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/articles/tutorials/manders-coeffients-in-cyteseer" />
      
      <id>tag:valasciences.com,2010:articles/f/4.188</id>
      <published>2010-02-12T21:08:50Z</published>
      <updated>2010-02-12T21:08:50Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <category term="Tutorials"
        scheme="http://www.valasciences.com/site/C6/"
        label="Tutorials" />
      <category term="Getting Started with CyteSeer Software"
        scheme="http://www.valasciences.com/site/C15/"
        label="Getting Started with CyteSeer Software" />
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    </entry>

    <entry>
      <title>Count Muscle Fibers Positive for a Particular Myosin Isoform</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/articles/tutorials/count-muscle-fibers-positive-for-a-particular-myosin-isoform" />
      
      <id>tag:valasciences.com,2009:articles/f/4.185</id>
      <published>2009-11-30T19:13:17Z</published>
      <updated>2009-11-30T19:13:17Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <category term="Tutorials"
        scheme="http://www.valasciences.com/site/C6/"
        label="Tutorials" />
      <category term="CyteSeer Examples"
        scheme="http://www.valasciences.com/site/C12/"
        label="CyteSeer Examples" />
      <content type="html"><![CDATA[
                
        
      ]]></content>
    </entry>

    <entry>
      <title>Custom Algorithm Pipelines in CyteSeer</title>
      <link rel="alternate" type="text/html" href="http://www.valasciences.com/articles/tutorials/custom-algorithm-pipelines-in-cyteseer" />
      
      <id>tag:valasciences.com,2009:articles/f/4.184</id>
      <published>2009-11-06T01:47:18Z</published>
      <updated>2009-11-26T07:32:52Z</updated>
      <author>
            <name>Vala Sciences</name>
            <email>info@valasciences.com</email>
            <uri>http://www.valasciences.com/</uri>
      </author>

      <category term="Tutorials"
        scheme="http://www.valasciences.com/site/C6/"
        label="Tutorials" />
      <category term="Getting Started with CyteSeer Software"
        scheme="http://www.valasciences.com/site/C15/"
        label="Getting Started with CyteSeer Software" />
      <content type="html"><![CDATA[
                
        
      ]]></content>
    </entry>


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