Cell-Cell Junctions

Vala Sciences provides High Content Screening (HCS) assays for the quantification of cell junction protein expression and localization.  Note that in addition to cadherin characterization, each assay below can be multiplexed to include beta-catenin and/or a custom biomarker.

VE-CAD

Cells: Human Neodermal Microvascular Endothelial Cells (HMVEC-nd)

Our high content screen quantifies the effect of test compounds on the expression levels of VE-Cadherin (VE-CAD), an integral membrane protein which establishes cell-cell junctions and critical to vascular permeability and development. This assay also visualizes the effects of test compounds on the cellular localization of VE-CAD.


E-CAD

Cells: MCF7 (human breast cancer cell)

Vala Sciences’ high throughput assay screen quantifies the effect of test compounds on the expression levels of E-Cadherin (E-CAD), an integral membrane protein essential to cell-cell adhesion and a known tumor suppressor whose expression is down-regulated during tumor progression. This assay quantifies test compound action on the up-regulation or down-regulation of E-CAD and images for cellular localization.